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resource source identifier human blca cell line rt4  (ATCC)


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    ATCC resource source identifier human blca cell line rt4
    Resource Source Identifier Human Blca Cell Line Rt4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/resource+source+identifier+human+blca+cell+line+rt4/RT4/pm39906564-830-2-13
    Average 96 stars, based on 792 article reviews
    resource source identifier human blca cell line rt4 - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human BLCA cell line RT4 (white male patient) ATCC HTB-2 TM Human BLCA cell line T24 (white female patient) ATCC HTB-4TM Recombinant DNA pRP[Exp]-Puro EF1A>hST6GALNAC1 [NM_018414.5] vector VectorBuilder VB190819-1167uwn pRP[Exp]-Puro-EF1A>(Stuffer_300bp) vector VectorBuilder VB191025-4255jjz Software and algorithms CXP Software Beckman Coulter Version 2.2 Excalibur Qual Browser ThermoFisher Scientific V2.2.44 Fiji on GitHub GPLv3+ MaxQuant Max-Planck-Institute of Biochemistry Version 1.6.17.0 Peak ScannerTM Software ThermoFisher Scientific Version 1.0 Phosphomatics Bio21 Molecular Science and Biotechnology Institute N/A Prism software GraphPad Version 10 R R Project for Statistical Computing Version 4.2.1 Other AG 50W-X8 Resin Bio-Rad 1431441 BioCoatTM Matrigel 24 well plates Corning CLS354480 Corning Matrigel Matrix Corning 356237 CycloscopeTM Cytognos CYT-CS-R-50 Mini-PROTEAN TGX Stain-Free Precast Gels 4-20%, 10 well comb, 50ul/well Bio-Rad 456-8094 MultiScreen-IP Filter Plate, 0.45 mm, clear, sterile Millipore MAIPS4510 .. Human BLCA cell lines RT4 (white male patient, HTB-2 TM), 5637 (white male patient, HTB-9TM), T24 (white female patient, HTB-4TM), and HT1197 (white male patient, CRL-1473 TM) were purchased from American Type Culture Collection (ATCC).

    Software:

    Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human BLCA cell line RT4 (white male patient) ATCC HTB-2 TM Human BLCA cell line T24 (white female patient) ATCC HTB-4TM Recombinant DNA pRP[Exp]-Puro EF1A>hST6GALNAC1 [NM_018414.5] vector VectorBuilder VB190819-1167uwn pRP[Exp]-Puro-EF1A>(Stuffer_300bp) vector VectorBuilder VB191025-4255jjz Software and algorithms CXP Software Beckman Coulter Version 2.2 Excalibur Qual Browser ThermoFisher Scientific V2.2.44 Fiji on GitHub GPLv3+ MaxQuant Max-Planck-Institute of Biochemistry Version 1.6.17.0 Peak ScannerTM Software ThermoFisher Scientific Version 1.0 Phosphomatics Bio21 Molecular Science and Biotechnology Institute N/A Prism software GraphPad Version 10 R R Project for Statistical Computing Version 4.2.1 Other AG 50W-X8 Resin Bio-Rad 1431441 BioCoatTM Matrigel 24 well plates Corning CLS354480 Corning Matrigel Matrix Corning 356237 CycloscopeTM Cytognos CYT-CS-R-50 Mini-PROTEAN TGX Stain-Free Precast Gels 4-20%, 10 well comb, 50ul/well Bio-Rad 456-8094 MultiScreen-IP Filter Plate, 0.45 mm, clear, sterile Millipore MAIPS4510 .. Human BLCA cell lines RT4 (white male patient, HTB-2 TM), 5637 (white male patient, HTB-9TM), T24 (white female patient, HTB-4TM), and HT1197 (white male patient, CRL-1473 TM) were purchased from American Type Culture Collection (ATCC).

    Staining:

    Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human BLCA cell line RT4 (white male patient) ATCC HTB-2 TM Human BLCA cell line T24 (white female patient) ATCC HTB-4TM Recombinant DNA pRP[Exp]-Puro EF1A>hST6GALNAC1 [NM_018414.5] vector VectorBuilder VB190819-1167uwn pRP[Exp]-Puro-EF1A>(Stuffer_300bp) vector VectorBuilder VB191025-4255jjz Software and algorithms CXP Software Beckman Coulter Version 2.2 Excalibur Qual Browser ThermoFisher Scientific V2.2.44 Fiji on GitHub GPLv3+ MaxQuant Max-Planck-Institute of Biochemistry Version 1.6.17.0 Peak ScannerTM Software ThermoFisher Scientific Version 1.0 Phosphomatics Bio21 Molecular Science and Biotechnology Institute N/A Prism software GraphPad Version 10 R R Project for Statistical Computing Version 4.2.1 Other AG 50W-X8 Resin Bio-Rad 1431441 BioCoatTM Matrigel 24 well plates Corning CLS354480 Corning Matrigel Matrix Corning 356237 CycloscopeTM Cytognos CYT-CS-R-50 Mini-PROTEAN TGX Stain-Free Precast Gels 4-20%, 10 well comb, 50ul/well Bio-Rad 456-8094 MultiScreen-IP Filter Plate, 0.45 mm, clear, sterile Millipore MAIPS4510 .. Human BLCA cell lines RT4 (white male patient, HTB-2 TM), 5637 (white male patient, HTB-9TM), T24 (white female patient, HTB-4TM), and HT1197 (white male patient, CRL-1473 TM) were purchased from American Type Culture Collection (ATCC).

    Sterility:

    Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human BLCA cell line RT4 (white male patient) ATCC HTB-2 TM Human BLCA cell line T24 (white female patient) ATCC HTB-4TM Recombinant DNA pRP[Exp]-Puro EF1A>hST6GALNAC1 [NM_018414.5] vector VectorBuilder VB190819-1167uwn pRP[Exp]-Puro-EF1A>(Stuffer_300bp) vector VectorBuilder VB191025-4255jjz Software and algorithms CXP Software Beckman Coulter Version 2.2 Excalibur Qual Browser ThermoFisher Scientific V2.2.44 Fiji on GitHub GPLv3+ MaxQuant Max-Planck-Institute of Biochemistry Version 1.6.17.0 Peak ScannerTM Software ThermoFisher Scientific Version 1.0 Phosphomatics Bio21 Molecular Science and Biotechnology Institute N/A Prism software GraphPad Version 10 R R Project for Statistical Computing Version 4.2.1 Other AG 50W-X8 Resin Bio-Rad 1431441 BioCoatTM Matrigel 24 well plates Corning CLS354480 Corning Matrigel Matrix Corning 356237 CycloscopeTM Cytognos CYT-CS-R-50 Mini-PROTEAN TGX Stain-Free Precast Gels 4-20%, 10 well comb, 50ul/well Bio-Rad 456-8094 MultiScreen-IP Filter Plate, 0.45 mm, clear, sterile Millipore MAIPS4510 .. Human BLCA cell lines RT4 (white male patient, HTB-2 TM), 5637 (white male patient, HTB-9TM), T24 (white female patient, HTB-4TM), and HT1197 (white male patient, CRL-1473 TM) were purchased from American Type Culture Collection (ATCC).



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    resource source identifier human blca cell line rt4  (ATCC)
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    ATCC resource source identifier human blca cell line rt4
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    https://www.bioz.com/product/resource+source+identifier+human+blca+cell+line+rt4/RT4/pm39906564-830-2-13
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    		  Buy from Supplier

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